jak2 inhibitor ag490 Search Results


jak2 inhibitor ag490  (Cosmo Bio USA)
90
Cosmo Bio USA jak2 inhibitor ag490
Jak2 Inhibitor Ag490, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+inhibitor+ag490/pm20207226-227-10-14?v=Cosmo+Bio+USA
Average 90 stars, based on 1 article reviews
jak2 inhibitor ag490 - by Bioz Stars, 2026-07
90/100 stars
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jak2 inhibitor ag490  (AbMole Bioscience)
90
AbMole Bioscience jak2 inhibitor ag490
The effect of matrine on cell cycle progression was determined by propidium iodide (PI) staining and flow cytometry. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l <t>AG490,</t> or a combination of matrine and AG490 for 24 h. (A) Cell cycle distribution on FLS after 24 h of drug treatment. (B) Histogram depicts the percentage of cells in G0/G1, S and G2/M phases of the cell cycle. Data are expressed as mean ± SD of three independent experiments. * P<0.05, ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.01 vs. the AG490 group.
Jak2 Inhibitor Ag490, supplied by AbMole Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+inhibitor+ag490/pmc05358712-57-6-10?v=AbMole+Bioscience
Average 90 stars, based on 1 article reviews
jak2 inhibitor ag490 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
inhibitors of janus kinase 2 (jak2) ag490  (Microm International GmbH)
90
Microm International GmbH inhibitors of janus kinase 2 (jak2) ag490
The effect of matrine on cell cycle progression was determined by propidium iodide (PI) staining and flow cytometry. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l <t>AG490,</t> or a combination of matrine and AG490 for 24 h. (A) Cell cycle distribution on FLS after 24 h of drug treatment. (B) Histogram depicts the percentage of cells in G0/G1, S and G2/M phases of the cell cycle. Data are expressed as mean ± SD of three independent experiments. * P<0.05, ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.01 vs. the AG490 group.
Inhibitors Of Janus Kinase 2 (Jak2) Ag490, supplied by Microm International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jak2+inhibitor+ag490/10__1161_slash_res__99__5__e18-672-31-34?v=Microm+International+GmbH
Average 90 stars, based on 1 article reviews
inhibitors of janus kinase 2 (jak2) ag490 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

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The effect of matrine on cell cycle progression was determined by propidium iodide (PI) staining and flow cytometry. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. (A) Cell cycle distribution on FLS after 24 h of drug treatment. (B) Histogram depicts the percentage of cells in G0/G1, S and G2/M phases of the cell cycle. Data are expressed as mean ± SD of three independent experiments. * P<0.05, ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.01 vs. the AG490 group.

Journal: International Journal of Molecular Medicine

Article Title: Matrine induces the apoptosis of fibroblast-like synoviocytes derived from rats with collagen-induced arthritis by suppressing the activation of the JAK/STAT signaling pathway

doi: 10.3892/ijmm.2016.2843

Figure Lengend Snippet: The effect of matrine on cell cycle progression was determined by propidium iodide (PI) staining and flow cytometry. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. (A) Cell cycle distribution on FLS after 24 h of drug treatment. (B) Histogram depicts the percentage of cells in G0/G1, S and G2/M phases of the cell cycle. Data are expressed as mean ± SD of three independent experiments. * P<0.05, ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.01 vs. the AG490 group.

Article Snippet: Cell treatments included the JAK2 inhibitor, AG490 (25 μ mol/l; Abmole Bioscience, Houston, TX, USA) or matrine (0.75 mg/ml; Aladdin Reagents Co., Ltd., Shanghai, China).

Techniques: Staining, Flow Cytometry, Isolation, Control, Cell Culture, In Vitro

The effect of matrine on apoptosis was determined by double staining with Annexin V-FITC/propidium iodide (PI) and flow cytometry. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. (A) Representative FACS scatter plots for each drug treatment. Q1 indicates necrotic cells; Q2 indicates late apoptotic cells; Q3 indicates early apoptotic cells; and Q4 indicates living cells. (B) Cell apoptosis rates were analyzed via flow cytometry. Data are presented as mean ± SD of three independent experiments. ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05 vs. the AG490 group.

Journal: International Journal of Molecular Medicine

Article Title: Matrine induces the apoptosis of fibroblast-like synoviocytes derived from rats with collagen-induced arthritis by suppressing the activation of the JAK/STAT signaling pathway

doi: 10.3892/ijmm.2016.2843

Figure Lengend Snippet: The effect of matrine on apoptosis was determined by double staining with Annexin V-FITC/propidium iodide (PI) and flow cytometry. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. (A) Representative FACS scatter plots for each drug treatment. Q1 indicates necrotic cells; Q2 indicates late apoptotic cells; Q3 indicates early apoptotic cells; and Q4 indicates living cells. (B) Cell apoptosis rates were analyzed via flow cytometry. Data are presented as mean ± SD of three independent experiments. ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05 vs. the AG490 group.

Article Snippet: Cell treatments included the JAK2 inhibitor, AG490 (25 μ mol/l; Abmole Bioscience, Houston, TX, USA) or matrine (0.75 mg/ml; Aladdin Reagents Co., Ltd., Shanghai, China).

Techniques: Double Staining, Flow Cytometry, Isolation, Control, Cell Culture, In Vitro

The effects of matrine on the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway were assessed by western blot analysis. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. (A) Western blot analysis of p-JAK2, p-STAT1 and p-STAT3 levels. β-actin was used as a loading control. (B) Quantitative densitometry of p-JAK2, p-STAT1 and p-STAT3 levels relative to β-actin. Data are presented as mean ± SD of three independent experiments. ** P<0.01 vs. the control group; # P<0.05, ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.05 vs. the AG490 group.

Journal: International Journal of Molecular Medicine

Article Title: Matrine induces the apoptosis of fibroblast-like synoviocytes derived from rats with collagen-induced arthritis by suppressing the activation of the JAK/STAT signaling pathway

doi: 10.3892/ijmm.2016.2843

Figure Lengend Snippet: The effects of matrine on the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway were assessed by western blot analysis. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. (A) Western blot analysis of p-JAK2, p-STAT1 and p-STAT3 levels. β-actin was used as a loading control. (B) Quantitative densitometry of p-JAK2, p-STAT1 and p-STAT3 levels relative to β-actin. Data are presented as mean ± SD of three independent experiments. ** P<0.01 vs. the control group; # P<0.05, ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.05 vs. the AG490 group.

Article Snippet: Cell treatments included the JAK2 inhibitor, AG490 (25 μ mol/l; Abmole Bioscience, Houston, TX, USA) or matrine (0.75 mg/ml; Aladdin Reagents Co., Ltd., Shanghai, China).

Techniques: Western Blot, Isolation, Control, Cell Culture, In Vitro

The effects of matrine on apoptotic markers were assessed by qRT-PCR. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. qRT-PCR analysis of relative expression of (A) Bax, (B) Bcl-2 and (C) caspase-3. (D) Bax to Bcl-2 ratio (based on relative intensity). In all experiments, β-actin was used as an internal control. Data are presented as the mean ± SD of three independent experiments. ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.01 vs. the AG490 group.

Journal: International Journal of Molecular Medicine

Article Title: Matrine induces the apoptosis of fibroblast-like synoviocytes derived from rats with collagen-induced arthritis by suppressing the activation of the JAK/STAT signaling pathway

doi: 10.3892/ijmm.2016.2843

Figure Lengend Snippet: The effects of matrine on apoptotic markers were assessed by qRT-PCR. Fibroblast-like synoviocytes (FLS) were isolated from control or collagen-induced arthritis (CIA) rats, cultured in vitro , and treated with 0.75 mg/ml matrine, 25 µ mol/l AG490, or a combination of matrine and AG490 for 24 h. qRT-PCR analysis of relative expression of (A) Bax, (B) Bcl-2 and (C) caspase-3. (D) Bax to Bcl-2 ratio (based on relative intensity). In all experiments, β-actin was used as an internal control. Data are presented as the mean ± SD of three independent experiments. ** P<0.01 vs. the control group; ## P<0.01 vs. the CIA group; Δ P<0.05, ΔΔ P<0.01 vs. the AG490 group.

Article Snippet: Cell treatments included the JAK2 inhibitor, AG490 (25 μ mol/l; Abmole Bioscience, Houston, TX, USA) or matrine (0.75 mg/ml; Aladdin Reagents Co., Ltd., Shanghai, China).

Techniques: Quantitative RT-PCR, Isolation, Control, Cell Culture, In Vitro, Expressing